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Brown, J, O’Brien, C.C., Lopes, A.C., Kolandaivelu, K., Edelman, E.R., 2018. Quantification of thrombus formation in malapposed coronary stents deployed in vitro through imaging analysis, Journal of Biomechanics, 71, 296-301.
Jonathan Brown, Project Manager, Edelman Lab (Harvard-MIT Biomedical Engineering Center):
“ScanIP was a huge help as it was a tool that allowed us not only to visualize our bench top sample results but also to extract quantitative data that would have otherwise been impossible to investigate.”
An in vitro flow loop setup was used to simulate blood flow conditions similar to those in a human coronary artery. Stents were deployed within the flow loops under a range of under expansion condition. Upon completion of the flow loop run samples was MicroCT scanned and the DICOM files were exported for processing in Simpleware ScanIP.
MicroCT data was imported into Simpleware ScanIP and pre-computed thresholding levels were used from prior experimental test to segment stent struts from clot formation and the fluid volume. Smoothing filters were further used to create continuous structures as part of a 3D visualization workflow. The Simpleware ScanIP API was later used to extract pixel valves for each mask for each MicroCT slice and plotted as an indication of clot formation. A custom MATLAB program was utilized to further extract strut position and calculate wall distances from mask pixel values on each slice.
Close-up of one of the stent struts reconstructed in Simpleware ScanIP software
The top three panels display Wall Distance vs. length along the vessel for each sample with red points indicating the median value for each microCT slice, and green points indicating the mean. The gray shaded region displays the 25%-75% interquartile range for each slice along the length of the vessel. The bottom panel displays clot formation vs. length along the vessel calculated as the number of pixel defined as clot over the total number of lumen defined pixels for each vessel
Phase lag suggests the fact that no clot is present on the struts but rather dispersed in between the struts