The most common closure system used to seal glass vials containing parenteral pharmaceuticals is an elastomer stopper held in place by a crimped aluminum band. This closure system is used to protect the contents against environmental factors including microbial contamination. X-ray micro-computed tomography (micro-CT) can provide detailed information regarding the position of the elastomer stopper and aluminum band both before and after the sealing process of parenteral vials. This information can be used to evaluate the closure integrity and identify if changes need to be made to the sealing process to ensure the integrity of the seal. Synopsys Simpleware software was used to accurately model and analyze the micro-CT data as part of a joint project with Micro Photonics.