Biofilm Preparation and Imaging
Biofilms were grown from Staphylococcus aureus, a clinical methicillin resistant (MRSA) strain of bacteria. Red and black colonies were selected and re-streaked out until a single black colony phenotype remained. Pieces of size 4 Ti-Cron™ braided polyester were cut into ~2-cm lengths and combined with the bacteria.
The suture and the biofilm were then imaged using confocal laser scanning microscopy (CLSM), specifically a Leica DM RXE upright microscope attached to a TCS SP2 AOBS confocal system. 3D image stacks were collected using different zoom settings and Z-slices.